遗传编码的荧光探针是解决活细胞、亚细胞、活体实时动态代谢技术监测难题的利器。杨弋教授和赵玉政教授研究团队过去聚焦这一代谢监测前沿领域，针对细胞内的关键辅酶NAD(H)、NADPH以及乳酸，经过高通量的筛选和优化，发展了系列特异性强、超级灵敏、快速响应的代谢物探针，在监测分析的分辨率、准确度、便捷性方面具有明显的优势，可谓是代谢分析的锐利武器。研究团队利用高性能代谢荧光探针，建立了原位、实时、动态的高通量分析方法，应用于活性化合物发现和基因筛选；利用多个遗传编码的荧光蛋白探针，研究团队首次在单细胞和活体水平建立了氧化还原 “全景式”实时动态分析技术，并应用于不同细胞器、细胞周期动态过程、巨噬细胞活化过程以及斑马鱼氧化还原状态研究。技术可推广到其他遗传编码的荧光探针，也可推广到多种分析平台。鉴于细胞代谢在生命过程和疾病诊疗中的重要性，利用荧光蛋白探针，对动物、细胞内代谢状态进行原位、实时动态的“全景式”检测和成像，为人们更好地理解物质与能量代谢的调节机制和代谢网络提供创新性的研究工具，也为相关疾病的诊断与创新药物发现提供重要技术支撑，对人类生命健康具有重要意义。相关研究发表在Cell、Nature子刊等国际权威学术期刊，成为该研究领域的标杆。
发展系列高性能遗传编码荧光探针
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